Flow cytometry stands as a crucial method in biological research with applications spanning across various disciplines, including microbiology, ecology, immunology, and clinical diagnostics. As a high-throughput single-cell method, it allows for the analysis of millions of cells with more than 30 parameters. It is also fundamental for sorting single cells for downstream analysis of defined cell subsets for single-cell RNAseq, etc. To be able to leverage flow cytometry to its full potential, an understating of the technology is necessary.
During Basics of Flow cytometry, students will attend theoretical lectures with follow-up practical hands-on sections that will cover all essentials of flow cytometry.
During the hands-on sessions held at the flow cytometry core facility of the Institute of Hematology and Blood Transfusion, students will have hands-on experience with advanced classical and spectral flow cytometers. They will be able to learn instrument setup. We plan to collaborate with flow cytometer manufacturers to also demo interesting instruments, that are not available in the core facility.